Your job is to match the remaining 11 chromosomes, determine whether or not your patient has a chromosomal abnormality and if so, which one, and to diagnose the patient with the expected symptoms of their karyotype. Scientists have developed several different tools and techniques for studying chromosomes, genes and base pairs in humans and other organisms. Karyotyping lab key north allegheny pdf book manual free. A chromosomal karyotype is used to detect chromosome abnormalities and thus used to diagnose genetic diseases, some birth defects, and certain disorders of the blood or lymphatic. Pdf biology 3201 karyotyping curriculum guide answers.
Students will cutout unmatched chromosomes and paste them next to their identical homologous mate. Karyotyping lab karyotyping labchapters 9, 11 academic biology 10dr. The characterization of the chromosomal complement of an individual or a species, including number, form, and size of the chromosomes. Lester md, phd, in manual of surgical pathology third edition, 2010. Use figure 3 on page 20 in the lab manual as a guide to help you with this exercise. Burran, susan and desrochers, david, principles of biology i lab manual. However, there are nevertheless many people who in addition to dont. A karyotype may be used to diagnose genetic diseases, some birth defects, such as down syndrome, or leukemia and lymphoma. Nov 19, 2019 a karyotype is the number and appearance of chromosomes. Karyotypes definition of karyotypes by the free dictionary. When their karyotype is finished they will be able to identify 1 of 3 different karyotype disorders. Patient b will be diagnosed with klinefelters syndrome. Clean up your materials and wash your hands before leaving the lab.
Find out what the test looks for and when its done. What is the diploid chromosome number for your karyotype. When finished, use the print screen option to copy your karyotype into a word processing document or image editor. Lab equipment worksheet answer key awesome lab equipment task cards biology and life science. Human karyotyping lab pages 1 9 text version fliphtml5. A karyotype can show prospective parents whether they have certain abnormalities that could be passed on to theiroffspring, or it may be used to learn the cause of a childs disability. Mar 01, 2012 this video will answer the following questions. Many alterations to the normal karyotype have been. Click on a chromosome, hold the mouse button down, and drag the selected chromosome onto the karyotype form. Karyotype lab displaying top 8 worksheets found for this concept some of the worksheets for this concept are lesson 2 analyzing chromosomes, 14, human karyotyping lab, biology 1 work i selected answers, karyotyping activity, appendix a human karyotyping work, karyotyping part 2, male chromosomes.
Ap biology fruit fly project biology 3201 unit 1 unit 2 unit 3. A karyotype can show prospective parents whether they have certain abnormalities that could be passed on to their. In this activity, you will use a computer model to look at chromosomes and prepare a karyotype. You will diagnose patients for abnormalities and learn the correct notation for characterizing karyotypes.
Karyotype definition of karyotype by the free dictionary. This important model can be broken down into four main ideas. A karyotype can show prospective parents whether they have certain abnormalities that could be passed on to theiroffspring, or it may be. Karyotyping is the process of pairing and ordering all the chromosomes of an. Karyotype also refers to the actual collection of chromosomes being examined. And with you in reality dependence a book to read, pick this karyotype paper lab as. The study of life bio life or living ology to study the knowledge obtained when scientists. In this karyotyping lesson plan, students analyze the traits and characteristics that can be determined from a karyotype such as the sex of the baby and particular traits identified by letters on the karyotype. Karyotyping lab point pleasant beach school district. To obtain a persons karyotype, laboratory technicians grow human cells in tissue culture media. A common misconception is that bands represent single genes. Ap biology fruit fly project biology 3201 unit 1 unit 2 unit 3 unit 4 work sheet. When their karyotype is finished they will be able to identify 1 of 3 different karyoty. Karyotype chromosome lab activity by beverly biology tpt.
How can a karyotype analysis detect genetic disorders. From crime scene forensics to blood typing, venture into the virtual laboratory and. University of alabama at birmingham cytogenetics laboratory. Which stage of the cell cycle would be a good point to perform a karyotype. Which sex chromosomes are present in the karyotype you prepared. The choices of the words, dictions, and how the author conveys the publication and lesson to the readers are enormously easy to understand. A karyotype is the number and appearance of chromosomes, and includes their length, banding pattern, and centromere position. Karyotyping lab lesson plan for 9th 10th grade lesson planet. A chromosomal karyotype is used to detect chromosome abnormalities and thus used to diagnose genetic diseases, some birth defects, and certain disorders of the blood or lymphatic system. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Read book biology karyotype lab answers rather than enjoying a good book with a cup of tea in the afternoon, instead they are facing with some infectious virus inside their laptop. The human karyotype biology homework just a random timelapse video i made.
Based upon your karyotype, determine the patients gender and the chromosomal abnormality of the patient. One normal male, one normal female, two different disorders of your choice out of the four. In the laboratory, the isolated cells are stimulated to begin actively dividing. Identification of chromosomes and karyotypes biology. A karyotype analysis usually involves blocking cells in mitosis and staining the condensed chromosomes with giemsa dye. A karyotype is a photograph of all of an organisms chromosomes. You will be arranging chromosomes into a completed karyotype and interpreting your findings jus. In a karyotype, chromosomes are arranged and numbered by size, from largest to smallest. Biology the appearance of the chromosomes in a somatic cell of an individual or species, with reference to their number, size, shape, etc. Some people with chromosomal disorders may have too many or too few chromosomes in their cells. Additional laboratory minutes may be granted for work above and beyond the four requir ed karyotypes.
In the laboratory, the isolated cells are stimulated to begin actively. A fetus, using amniotic fluid or chorionic villi tissue from the placenta. In humans, in every cell of our bodies, we typically will have 23 pairs of chromosomes. A karyotype is an organized profile of a persons chromosomes. Karyotyping is a laboratory procedure that allows your doctor to examine your set of chromosomes. From each pair, we typically will inherit one chromosome from our mother and one from our father. Is the fetus represented by your karyotype male or female.
Karyotype chromosome lab activity activities, edwards. For each couple, you must construct and analyze a karyotype. The chromosomal composition of each species has a number of characteristics. Biology 2970 computer laboratory human karyotype nslc. You can publish your book online for free in a few minutes.
We also acknowledge previous national science foundation support under grant numbers 1246120, 1525057. Summarize how a karyotype is made in 5 major steps. So, you can gate human karyotype lab answer key easily from some device to maximize the technology usage. All species are characterized by a set of chromosomes to carry their genetic information.
Making a karyotype, an online activity from the university of utahs genetic science learning center. Printable karyotype project from biology corner, a resource site for biology and science teachers. Karyotyping activity with case histories from the university of arizonas biology project. Karyotype, karyotyping and preparation of idiogram what is a karyotype. Whats the difference between an autosome and a sex chromosome. A karyotype is a picture in which the chromosomes of a cell have been stained so that the banding pattern of the chromosomes is visible. After being stained and sorted, the chromosomes are counted and displayed. Here you will find all we have for karyotyping worksheet.
Biology the appearance of the chromosomes in a somatic cell of an. A karyotype depicts the number, size, and any abnormalities of the. In addition, the book offers an informative tutorial on basic laboratory procedures including microscopy, photomicrography, automation, and computerized karyotyping, reports on such novel synergistic. They can tell us everything from the likelihood that an unborn baby will have a genetic disorder to whether a person will be male or female. You will be arranging chromosomes into a completed karyotype, and interpreting your findings just as if you were working in a genetic analysis program at a hospital or clinic. A karyotype is the number and appearance of chromosomes, and includes their length, banding pattern. This exercise is a simulation of human karyotyping using digital images of chromosomes from actual human genetic studies. This karyotyping lab lesson plan is suitable for 9th 10th grade. The biology project university of arizona the biology project, human biology karyotyping in spanish dna activity in spanish dna profiling in. Not many karyotyping activities provide you this many sets. The isolation and microscopic observation of chromosomes forms the basis of cytogenetics and is the primary method by which clinicians detect chromosomal abnormalities in humans. In addition to changes in whole chromosome number, discussed in the following section, a variety of subchromosomal alterations are possible. Cells in metaphase of cell division are stained to show the distinct parts of the chromosomes. The cells are obtained from the blood, skin, or bone marrow or by amniocentesis or chorionic villus sampling, as noted above.
Your doctor may suggest that you get a karyotype test, based on the results of a pregnancy screening test. This is a great activity where students complete a paper karyotype so they can diagnose a chromosome disorder. The following procedure is utilized to perform this laboratory experience. Step 1 practice the first karyotype, this one should be normal. This notation includes the total number of chromosomes, the sex chromosomes, and any extra or missing autosomal. This is a diverse set of karyotypes 12 different sets of idiogram chromosomes. Pay attention to chromosome size, centromere location, and banding patterns. Karyotypes the isolation and microscopic observation of chromosomes forms the basis of cytogenetics and is the primary method by which clinicians detect chromosomal abnormalities in humans. Karyotyping lab key north allegheny pdf book manual. Many alterations to the normal karyotype have been generated and examined. From crime scene forensics to blood typing, venture into the virtual laboratory and conduct your own investigations. Using the attached sheets, complete four different karyotypes. In making a karyotype, what are three characteristics of the chromosomes that can be used to pair homologues. Karyotyping activity introduction this exercise is a simulation of human karyotyping using digital images of chromosomes from actual human genetic studies.
Lab technicians compile karyotypes and then use a specific notation to characterize the karyotype. Karyotyping activity the biology project university of arizona. This allows students to create karyotypes for 2 normal karyotypes male and female plus a va. Principles of biology i lab manual galileo open learning. What is the haploid chromosome number for your karyotype your karyotype may have 2 answers here. How do two physically healthy parents produce a child with down syndrome and a second child with cri du chat syndrome. Comets living environment laboratory manual, 20052006, south lewis high school, turin, new york. Scientists often analyze chromosomes in prenatal testing and in diagnosing specific diseases. When we talk concerning karyotype worksheet answers biology, we already collected several related images to inform you more. Lab 14 karyotyping with magnetic chromosomes ap biology. Introduction mendels model of inheritance is the basis for modern genetics. What three things can a karyotype tell a biologist. The figure on the back of this page is a representation of gbanded chromosomes only 1 chromosome from each homologous pair is represented.
To obtain a view of an individuals karyotype, cytologists photograph the chromosomes and then cut and paste each chromosome into a chart, or karyogram, also known as an ideogram. Through karyotypes, it is often possible to see genetic disorders such as downs syndrome simply by looking at a karyotype. A karyotype analysis can be used to look at the number and appearance of chromosomes in individuals. Thakker, in genetics of bone biology and skeletal disease second edition, 2018. The human male determines the sex of all of his offspring. This is a handson paper lab where students create a karyotype. This arrangement helps scientists quickly identify chromosomal alterations that may result in a genetic disorder.
What was the sex of the person in the karyotype that you constructed. Step 2 go to next page to diagnose an individual from one of the case studies. Analysis questions 1 how many autosomes any chromosome that that is not a sex chromosome are in a normal human karyotype. Karyotype analysis flashcards and study sets quizlet. The karyotype is a set of characteristics that identifies and describes a particular set of chromosome. Read online answers to human genome karyotype biology lab as release as you can discover the key to count the lifestyle by reading this answers to human genome karyotype biology lab this is a nice of compilation that you require currently.
Modern biology 80 datasheets for intext labs name class date analyzing karyotypes continued 8. The dye stains regions of chromosomes that are rich in the base pairs adenine a and thymine t producing a dark band. Karyotyping lab lesson plan for 9th 10th grade lesson. Choose from 49 different sets of karyotype analysis flashcards on quizlet. Refer to pages 188208 in your russell textbook for descriptions of the abnormalities. Karyotype analysis of human chromosome easybiologyclass. Color genetic makeup gender karyotype disorder blue 46 xx f normal na purple 47 xx f abnormal down syndrome green 47 xy m abnormal trisomy 21 tan 47 xy m abnormal klinefelter syndrome pink 46 xy m normal na orange 45 xx m abnormal turner syndrome ap biology laboratory 14. Karyotyping lab aneuploidy is a change in the number of chromosomes that can lead to a chromosomal abnormality. Chromosome analysis karyotyping at a glance why get tested.
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